Beer fermentation: the phases of the curve in a 2024 study

A study by the University of Salerno and the University of Brescia describes the three phases of beer fermentation and what happens with less yeast. The extract curve in MicroDens Logger Pro.

A study published in 2024 in the journal Fermentation by researchers at the University of Salerno and the University of Brescia followed the fermentation of six types of beer in a microbrewery and of two batches in the laboratory.

Three phases

The authors divide the alcoholic fermentation of beer into three phases, each recognizable from the rate at which the process advances.

1LagThe yeast adapts to the wort, absorbs nutrients and prepares for cell division. From 5–6 hours up to 24 hours, depending on the recipe.
2Exponential phaseThe yeast grows rapidly and fermentation reaches its highest rate.
3Stationary phaseYeast reproduction stops for lack of nutrients and fermentation comes to an end.

The production trials took place in a microbrewery in the province of Salerno, in 150 L cylindrical fermenters with 90 L batches, in a fermentation room at 16 °C. The six beers differed in recipe and original gravity. In all the batches, followed for six days, the three phases were recognizable.

STUDY DATA

The beers in the study

Original gravity (OG) and final gravity (FG) of the wort in °Plato, calculated from OG and FG with the ASBC formula; in brackets the specific gravity reported in the study, measured with a density meter with 0.001 g/cm³ resolution.

BeerOGFG
Lemon Ale9.0 °P (1.036)2.6 °P (1.010)
British Golden Ale11.2 °P (1.045)2.6 °P (1.010)
Apple Wit Ale11.9 °P (1.048)2.6 °P (1.010)
Belgian Pale Ale13.3 °P (1.054)3.6 °P (1.014)
English IPA14.3 °P (1.058)3.1 °P (1.012)
British Strong Ale17.7 °P (1.073)4.6 °P (1.018)

The same wort with less yeast

In the laboratory the authors set up two fermentations with the same wort, made from 4 kg of liquid malt extract and 20 L of water, with an original gravity of 13.3 °P (OG 1.054) and the same yeast strain. Only the amount of yeast pitched was different.

Reference batch

22 g

Known recipe, brewed according to good brewing practice. Final gravity reached: 3.6 °P (FG 1.014).

13.3 °Psame wort, OG 1.054

Under-pitched batch

6 g

Markedly slower fermentation. The final gravity was not reached: fermentation remained incomplete.

For the authors this is an example of how the course of fermentation, observed while it happens, shows whether a batch is progressing as it should. Among the next steps they mention alarm thresholds, derived from data on new recipes, to flag a problem to the brewmaster in good time.

Fermenter weight and wort density

The study starts from an observation about the sector: microbreweries are mostly small companies that often cannot afford the monitoring systems built into the plants of industrial breweries, yet they still need to control the process to keep the quality of their beer consistent.

The proposed system weighs the fermenter. During fermentation the yeast converts sugars into alcohol and carbon dioxide, which escapes through the airlock on the lid: the weight drops, and the rate at which it drops distinguishes the three phases. To compare weight and density, in one laboratory trial the researchers took samples from the fermenter during the process and measured their density with a triple-scale hydrometer, finding a linear relationship between the two.

The extract curve

With traditional methods, the authors note, fermentation is followed by periodic sampling. With a density meter, the curve is built from the density of the samples: the original gravity of the wort is the starting point, and the apparent extract in °Plato falls during fermentation and settles at the final gravity.

Each reading on the fermenter curve

The MicroDens portable density meter follows fermentation with the BeerQuick method, without distillation: the wort reading sets the original gravity, and each subsequent reading of the fermenting beer updates the estimates of alcohol and attenuation.

MicroDens Logger Pro, Fermentation module

In MicroDens Logger Pro, the Gibertini Elettronica software for the MicroDens density meter, the Fermentation module plots each BeerQuick reading on the curve of the fermenter with the same original gravity. The apparent extract curve runs from the OG of the wort to the final gravity and shows the lag, active fermentation, slowdown and end phases. The current batch can be compared with up to five earlier batches of the same method, with the curves aligned from the start; from the curve of a reference batch or from the trend of the last 24 hours the software estimates the final gravity and the remaining time, with a confidence rating.

°P

Apparent extract, from OG to final gravity

4 phases

Lag, active fermentation, slowdown, end

5 batches

Compared with the current one

±0.0001 g/cm³

Density accuracy, MicroDens

MicroDens for beer

Last updated: November 3, 2026